Ion Torrent Ion PGM™ Sequencer

Instrument Details

• Make : Life technologies, USA
• Model : Ion Torrent Ion PGM™ Sequencer 

Specifications :

• Capacity: 1 chip per run
• Chip Compatibility: Ion314TM Chip v2 BC, Ion  316TM Chip v2 BC, Ion 318TM Chip v2 BC
• Comments:Compatible with Ion OneTouch™ automated template prep system
• Data Output: BAM Files, FASTQ Files, SFF Files, VCF Files
• Dimensions: 24 in (61 cm) (W) x 20 in (51 cm) (D) x 21 in (53 cm) (H)
• For Use With (Equipment): Ion PGM™ System
• High Throughput Compatibility: High Throughput-Compatible, Multiplexing
• Output:Ion 314™ Chip v2: Up to 100 Mb, Ion 316™ Chip v2: Up to 1 Gb, Ion 318™ Chip v2: Up to 2 Gb
• Product Line: Ion Torrent™, Personal Genome Machine (PGM™)
• Product Size:1 system
• Read Length: 35–400 bases (average: 200 bases)
• Reads⁄Run:Ion 314™ Chip v2: 400-500 thousand, Ion 316™ Chip v2: 2-3 million, Ion 318™ Chip v2: 4-5.5 million
• Run Time:Ion 314™ Chip v2: 2.3 to 3.7 hr, Ion 316™ Chip v2: 3.0 to 4.9 hr, Ion 318™ Chip v2: 4.4 to 7.3 hr
• Technique:Post-Light™ Ion Semiconductor Sequencing
• Throughput: Up to 2 Gb per run
• Weight: 65 lbs (30 kg) (Freestanding) 

Working Principle: Genetic Analyzer: Capillary Electrophoresis OverviewThe Ion Personal Genome Machine (PGM) System combines semiconductor sequencing technology with natural biochemistry to directly translate chemical information into digital data, democratizing sequencing and making it accessible to your lab. This established system leverages the exponential improvements in the semiconductor industry (known as Moore’s Law) to provide scalability and flexibility for various applications. The Ion PGM System uses natural sequencing chemistry, which eliminates the need for expensive optics and reduces complex chemistries to measure natural DNA extension. The system leverages direct, real-time sequencing detection, providing sequencing results typically in 3–7 hours.Ion Torrent™ Technology directly translates chemically encoded information (A, C, G, T) into digital information on a semiconductor chip. Simple natural chemistry helps result in unmatched uniformity of coverage. Ion Torrent™ uses simple sequencing chemistry including natural nucleotides, no enzymatic cascade, no fluorescence, no chemiluminescence, no optics, no light: The Chip is the Machine. The Ion PGM Sequencer has 3 chip densities for optimal performance. The Ion PGM system is ideal for small genome sequencing, targeted DNA and RNA sequencing when coupled with Ion AmpliSeq technology. Ion AmpliSeq™ technology delivers simple and fast library construction for affordable targeted sequencing of specific human genes or genomic regions. Based on ultrahigh-multiplex PCR, Ion AmpliSeq™ technology requires as little as 10 ng of input DNA to target sets of genes. There are three AmpliSeq Panels to choose from: Ready-to-use, Custom Panels and Community Panels.

Main Features of the Instrument

• Rapid and Simple Assay Set-Up
• Sensitive LED-Based 4 Color Fluorescence Reading
• Compatible with Many Genomic Analysis Techniques
• SNP Genotyping 

Applications:

• DNA Sequencing of microbial pathogens|
• Epigenetic Sequencing of plant varieties
• Fragment Analysis
• Genotyping &Meta Genomic Profiling
• RNA Sequencing and small RNA profiling of plant pathogens

Contact Us:

• Director, ICAR-CPRI,Shimla(H.P.)-171001
• Phone: 0177-2625073
• Mobile:+91-9418657678
• Email Id: director[dot]cpri[at]icar[dot]gov[dot]in