3500 Genetic Analyzer

Instrument Details

• Make : Hitachi Applied Biosystems
• Model : 3500 Genetic Analyzer 8 CH RUO

Specifications :

• Capillary Length: 36 cm, 50 cm
• Compatible Polymers: POP-4™, POP-6™, POP-7™
• Compatible Products: 3500 Genetic Analyzer Capillary Array (36 cm), 3500 Genetic Analyzer Capillary Array (50 cm), POP-4™ Polymer for 3500⁄3500xL Genetic Analyzers, POP-6™ Polymer for 3500⁄3500xL Genetic Analyzers, POP-7™ Polymer for 3500⁄3500xL Genetic Analyzers
• Current Limit: 15 amps
• External Computer: Hard Drive: 2x 500GB SATA 3.0 Gb/s and 8 MB Data Burst Cache, Memory: 16 GB (2x 8GB) 1600 MHz DDR3 Non-ECC. 4th Gen Intel Core I7 Processor- 3.1 GHz Turbo Processor, Operating System: Windows 7 SP1
• For Use With (Equipment): 3500 Genetic Analyzer
• Format: 8-well strips, 96-well plate
• Number of Capillaries: 8 Capillaries
• Operating Environment: Humidity: 20%-80% (non-condensing), Room temp: <2° fluctuation during runs, Temperature: 15°C-30°C
• Operating System: Windows 7 (Service Pack 1)
• Plan Duration: 1 Year Limited Warranty on Parts and Labor, Service Installation and Application Training Included
• Polymer Consumption: Replace at earliest of 7 days⁄384 samples⁄60 Injections (384-sample pouches), Replace at earliest of 7 days⁄960 samples⁄120 Injections (960-sample pouches)
• Product Size: 1 system
• Read Length: ≥850 bp using a 50 cm capillary and POP-7™
• Throughput: Up to 424 samples per day (using run module HID36 POP-7)
• Voltage Limit: Electrophoresis Voltage – Up to 20 kV

Working Principle: Genetic Analyzer: Capillary Electrophoresis Overview

In the theory, capillary electrophoresis occurs when an electric field (E) is applied to an electrolyte solution within a capillary, causing ions to migrates

• Driving force on ions is the applied electrical field to the electrolyte within a capillary
• DNA fragments are separated as they migrate by size exclusion Sieving effect
• Dye labeled fragments detected by fluorescence and laser and rendered into the sequenceIn the theory

Applications:

• DNA fragment Sequencing
• Fragment Analysis (Genetic fidelity test)
• Genotyping & Genomic Profiling
• Sanger Sequencing

Contact Us:

• Director, ICAR-CPRI,Shimla(H.P.)-171001
• Phone: 0177-2625073
• Mobile:+91-9418046415
• Email Id: director.cpri@icar.gov.in