96 wells StepOnePlus™ Real-Time PCR System

Instrument Details

• Make : Applied Biosystems Inc, USA
• Model : StepOnePlus™ Real-Time PCR System 
  
  

Specifications :

• Ability to perform high-resolution melt analysis (additional software package required)
• Cost-effective 4-color/96-well system deliver precise, quantitative real-time PCR results
• Long-life, LED-based optical system records fluorescence from FAM™/SYBR® Green I, VIC™/JOE™, and ROX™ dyes for gene expression analysis, pathogen quantitation, SNP genotyping, and presence/absence assays. The StepOnePlus system also accommodates the TAMRA™ dye.
• The system perform Fast PCR reactions in less than 40 minutes
• Ultra-compact footprint fits any laboratory setting
• LCD touch screen and USB drive provide configuration flexibility and enable PC-free operation
• Remote monitoring and email notification for convenience and time savings
• The StepOnePlus system features VeriFlex Block technology, which combines six independently controllable Peltier blocks for enhanced PCR functionality and precise temperature control.
   
  

Working Principle:System is equipped with fluorophore detection chemistries that include FAM and VIC dye–labeled Applied Biosystems™ TaqMan™ MGB probe-based assays, VIC and TAMRA dye–labeled probe-based assays, and SYBR Green I dye chemistry. [note: The TAMRA dye is available only with the StepOnePlus system.] TaqMan probe-based assays provide outstanding specificity and sensitivity, and SYBR Green I dye chemistry is an economical alternative for target identification, initial screening assays, or assays that require only a few reactions.

The StepOnePlus system use fluorescent-based polymerase chain reaction (PCR) reagents to provide:

• Quantitative detection of target nucleic acid sequences (targets) using real-time analysis.
• Qualitative detection of targets using post-PCR (endpoint) analysis.
• Qualitative analysis of the PCR product (achieved by melt curve analysis that occurs post-PCR).
• The system collect raw fluorescence data at different points during a PCR, depending on the type of run that the instruments perform. 
  
  

Main Features of the Instrument

• Rapid and Simple Assay Set-Up
• Sensitive LED-Based 4 Color Fluorescence Reading
• Compatible with Many Genomic Analysis Techniques
• SNP Genotyping

Application

• Gene Expression Analysis
• MicroRNA Expression
• Protein Expression
• Translocation Analysis
• Gene Detection
• Viral Load Analysis
• SNP genotyping
• Translocation analysis

Contact Us:

• Director, ICAR-CPRI,Shimla(H.P.)-171001
• Phone: 0177-2625073
• Mobile:+91-9418657678
• Email Id: director.cpri@icar.gov.in